2021/2022

2021/2022

DM270

BIO/11 (MOLECULAR BIOLOGY)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

EXPERIMENTAL AND APPLIED BIOLOGY

Scienze biomediche molecolari

1°

2nd semester (01/03/2022 - 14/06/2022)

6

48 lesson hours

Italian

ORAL TEST

MONTECUCCO ALESSANDRA (titolare) - 6 ECTS

Students should be aware of the fundamental of molecular biology: structure of nucleic acids and proteins, general mechanisms of DNA replication, transcription, translation, and techniques of molecular biology.

Understanding the molecular mechanisms controlling cell cycle progression and the cellular response to genotoxic stress.

This course concerns the molecular mechanisms underlying cell cycle control in eukaryotic cells. Main topics are:
- Model systems to study cell cycle control: yeasts, X. laevis, mammalian cells.
- Mechanisms that control cell cycle progression: genetic and epigenetic control of DNA replication and genome segregation, cell cycle checkpoints, DNA damage response and cell senescence.
- Cell death: apoptosis, autophagy, necrosis.
- Functional organization of cell nucleus throughout cell cycle and in response to cell stress.
- Chromatin organization: the histone code.
- Gene targeting and genome editing: homologous recombination, Zn-finger nucleases, CRISPR/Cas9 system. siRNA-mediated down-regulation of gene expression.
- Protein networks: two hybrid system, FRET, FRAP.

Lectures and seminars. If necessary, due to pandemic, lectures will be online

- Molecular Biology of the Cell (Sixth Edition) Albert B. et al.
- Essential Cell Biology, B. Alberts et al. 5th Edition Norton & C.
- Molecular Biology of the Gene, Watson, Baker, Bell, Gann, Levine, Losick, 7th edition.

Oral

Oral