Enrollment year
2017/2018
Academic discipline
BIO/11 (MOLECULAR BIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Curriculum
PERCORSO COMUNE
Period
1st semester (01/10/2017 - 14/01/2018)
Lesson hours
48 lesson hours
Prerequisites
Basic concepts in Molecular Biology, Genetics and Biochemistry
Learning outcomes
The objective of the course is to provide advanced knowledge in advanced molecular biology methods, including genomics and post-genomics. In addition, students will learn the english terminology in the field. Through journal clubs and scientific paper reading with the teacher, students will learn to critically evaluate the scientific literature in the field.
Course contents
Specific subjects include: organization of different genomes; DNA markers; genome maps; sequencing of entire genomes; next generation sequencing methods and applications; genome sequence interpretation (sequence analysis, experimental approaches, gene identification); defining gene function (gene inactivation, RNAi); genome modification by engineered nucleases; chromosome architecture (chromatin, centromeres, telomeres); comparative genomics; molecular mechanisms of genome evolution. Model organisms. Production of recombinant proteins.
Teaching methods
Lectures, Journals clubs, Discussion with students
Reccomended or required readings
"Genomes 3", TA Brown, Garland Science Publishing
"From genes to genomes, Concepts and applications of DNA technology", JW Dale, MV Schantz, N Plant, third edition, Wiley-Blackwell
"Gene cloning and DNA analysis, An Introduction" Fifth edition, TA Brown, Blackwell Publishing
Assessment methods
Written exam icluding open questions on general topics and multiple choice questions.
Further information
Written exam icluding open questions on general topics and multiple choice questions.
Sustainable development goals - Agenda 2030
Università di Pavia - Offerta formativa