2017/2018

2017/2018

DM270

BIO/11 (BIOLOGIA MOLECOLARE)

DIPARTIMENTO DI BIOLOGIA E BIOTECNOLOGIE "LAZZARO SPALLANZANI"

MOLECULAR BIOLOGY AND GENETICS

PERCORSO COMUNE

1°

Primo Semestre (01/10/2017 - 14/01/2018)

6

48 ore di attività frontale

English

ORALE

GIULOTTO ELENA (titolare) - 4.5 CFU
SULLIVAN KEVIN FRANCIS - 1.5 CFU

Basic concepts in Molecular Biology, Genetics and Biochemistry

The objective of the course is to provide advanced knowledge in advanced molecular biology methods, including genomics and post-genomics. In addition, students will learn the english terminology in the field. Through journal clubs and scientific paper reading with the teacher, students will learn to critically evaluate the scientific literature in the field.

Specific subjects include: organization of different genomes; DNA markers; genome maps; sequencing of entire genomes; next generation sequencing methods and applications; genome sequence interpretation (sequence analysis, experimental approaches, gene identification); defining gene function (gene inactivation, RNAi); genome modification by engineered nucleases; chromosome architecture (chromatin, centromeres, telomeres); comparative genomics; molecular mechanisms of genome evolution. Model organisms. Production of recombinant proteins.

Lectures, Journals clubs, Discussion with students

"Genomes 3", TA Brown, Garland Science Publishing

"From genes to genomes, Concepts and applications of DNA technology", JW Dale, MV Schantz, N Plant, third edition, Wiley-Blackwell

"Gene cloning and DNA analysis, An Introduction" Fifth edition, TA Brown, Blackwell Publishing

Written exam icluding open questions on general topics and multiple choice questions.

Written exam icluding open questions on general topics and multiple choice questions.