Enrollment year
2017/2018
Academic discipline
BIO/11 (MOLECULAR BIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
EXPERIMENTAL AND APPLIED BIOLOGY
Curriculum
Scienze biomediche molecolari
Period
2nd semester (01/03/2018 - 14/06/2018)
Lesson hours
48 lesson hours
Course contents
This course concerns the molecular mechanisms underlying cell cycle control in eukaryotic cells. Model systems to study cell cycle control: yeasts, X. laevis, mammalian cells. Mechanisms that control cell cycle progression: genetic and epigenetic control of DNA replication and genome segregation, cell cycle checkpoints, DNA damage response and cell senescence. Cell death: apoptosis, autophagy, necrosis. Functional organization of cell nucleus throughout cell cycle and in response to cell stress. Chromatin organization: the histone code. Gene targeting: homologous recombination, Zn-finger nucleases, CRISPR/Cas9 system. siRNA-mediated down-regulation of gene expression. Protein networks: two hybrid system, antibody arrays, FRET, FRAP. Next-generation Sequencing (NGS): protocols and applications.
Teaching methods
Lectures
Reccomended or required readings
=- Molecular Biology of the Cell (Sixth Edition) Albert B. et al.
- Molecular Biology of the Gene, Watson, Baker, Bell, Gann, Levine, Losick, 7th edition.
Assessment methods
= Oral
Further information
= Oral
Sustainable development goals - Agenda 2030
Università di Pavia - Offerta formativa