RATIONAL DESIGN IN MODERN BIOTECHNOLOGY: FROM CONCEPT TO CLINIC
Stampa
Enrollment year
2022/2023
Academic year
2023/2024
Regulations
DM270
Academic discipline
BIO/11 (MOLECULAR BIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
ADVANCED BIOTECHNOLOGY
Curriculum
PERCORSO COMUNE
Year of study
Period
1st semester (25/09/2023 - 15/01/2024)
ECTS
3
Lesson hours
24 lesson hours
Language
English
Activity type
ORAL TEST
Teacher
D'ANGELO IGOR EDMONDO PAOLO (titolare) - 3 ECTS
Prerequisites
Bachelor-level knowledge of molecular biology and chemistry.
Learning outcomes
Learning the fundamental concepts of structure-based drug development and applying them to case studies.
Course contents
Introduction to the pharmaceutical development process (from concept to the clinic).
Small vs large molecule biotherapeutics, pros and cons and applicability.
Antibodies and foundational immunology.
Antibody discovery: approaches to generate repertoires (incl. hybridoma, mammalian cell, in vitro and ML-based).
In vivo vs in vitro antibody libraries, options and limitations.
Antibody rational engineering and optimization: demo of the Abacus suite for sequence/structure based optimization via Machine Learning.
Antibody rational engineering and optimization: liabilities and immunogenicity.
Structure based rational design: introduction and demo utilizing the MOE or Schrodinger suite.
Rational design and engineering of affinity and stability.
Example studies of rational design in biotherapeutics: antibodies, antibody-like and new approaches.
Integrating deep sequencing for antibody engineering.
Career options in the pharmaceutical development field, pros and cons and discussion.
Teaching methods
Lectures; practice exercises to use databases and apply target structure analysis; round tables between teacher and students to further delve into topics addressed in the lectures and into more general themes about working in a biotech company.
Reccomended or required readings
Slides and other material provided by the teacher.
Assessment methods
Mandatory attendance of lectures and interactive activities (no final exam).
Further information
none
Sustainable development goals - Agenda 2030
The rational design of biotherapeutics, in particular relatively to antibody engieering, matches goal n. 3 of the Agenda 2030 for the sustainable development: "Good Health and Well-being" (3.B Support the research and development of vaccines and medicines for the communicable and noncommunicable diseases).
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