Enrollment year
2022/2023
Academic discipline
BIO/18 (GENETICS)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Curriculum
PERCORSO COMUNE
Period
(01/03/2023 - 15/06/2023)
Lesson hours
48 lesson hours
Prerequisites
Basic knowledge in genetics, cellular and molecular biology
Learning outcomes
It intends to provide a detailed technological overview of the most relevant and innovative methodologies in the field of neuroscience
Course contents
Multiparameter analysis using machine learning approaches and predictive nomograms
Protein misfolding amplification
flow cytometric analysis
Relative and absolute gene expression by Real time PCR
Experimental design of Taqman systems for gene expression analysis
Essays on the functionality of subcellular organelles
Essays on cellular functionality: vitality and proliferation
Essays on apototic cell death
Essays on the assessment of the autophagic process
Antisense DNA and gene interference by anti-microRNA
Protein expression analysis: immunoblotting and histoblotting
Evaluation of gene methylation
Gene transfection methods: lipofectamine, electroporation
Extracellular vesicle isolation and analysis procedures
Teaching methods
Frontal lessons in presence and in streaming
Reccomended or required readings
There are currently no reference texts available. Otherwise, information will be provided to students. Instead, all the slides of the lessons will be provided
Assessment methods
Exam consisting of an oral test on the contents
Sustainable development goals - Agenda 2030
Università di Pavia - Offerta formativa