Enrollment year
2021/2022
Academic discipline
MED/46 (LABORATORY MEDICINE TECHNIQUES)
Department
DEPARTMENT OF MOLECULAR MEDICINE
Course
BIOMEDICAL LABORATORY TECHNIQUES
Curriculum
PERCORSO COMUNE
Period
(03/10/2022 - 20/01/2023)
Lesson hours
32 lesson hours
Learning outcomes
1) Know the techniques of fixation and inclusion of histological samples and the problems corelated to the pre-analytical phase.
2) Know the technical principles underlying the main histochemical stains used in pathological anatomy
3) Know the main practical applications of histochemical staining.
4) Know and describe the main immunohistochemical staining techniques
5) Describe the main practical applications of immunohistochemistry in diagnostic pathology.
Course contents
1) Histological samples fixation and paraffin embedding techniques in surgical pathology lab
2) Routinary histological stainings
3) Special histochemical stainings (Pas, Alcina blue, PAS-Alcian, Congi Red etc): technical principles and practical applications in surgical pathology
4) Immunohistochemistry: history and general principles
5) Primary antibodies and antisera
6) Immunohistochemical stainings detection methods and signal enhancement techniques
7) Antigenic demasking techniques in immunohistochemistry
8) Automation in immunohistochemistry
9) Principles of diagnostic immunohistochemistry
11) Immunohistochemical characterization of the principal breast cancer prognostic and predictive factors
12) Quality control and monitoring of the pre-analytic phase.
Teaching methods
Lectures and presentation of practical application of the different histochemical and immunohistochemical techniques. Professor is available for clarification on the topics of the program.
Reccomended or required readings
Robbins, Pathological Bases of Diseases.
Assessment methods
Oral exam with multiple questions aimed at ascertaining the knowledge acquired on the main histologic, histochemical and immunohistochemical techniques and their practical application.
The assessment will also take into account the ability to communicate and use an appropriate scientific language.
Sustainable development goals - Agenda 2030