2021/2022

2021/2022

DM270

BIO/10 (BIOCHEMISTRY)

DEPARTMENT OF MOLECULAR MEDICINE

BIOMEDICAL LABORATORY TECHNIQUES

PERCORSO COMUNE

1°

(04/10/2021 - 21/01/2022)

2

16 lesson hours

Italian

ORAL TEST

CAMPAGNOLI MONICA - 2 ECTS

Knowledge of basic chemistry and biochemistry

Students are provided with knowledge on the main techniques used in biochemical laboratories for the purification and characterization of biological molecules. In particular, the course focuses on learning the basic theoretical and practical principles of spectroscopic, chromatographic and electrophoretic techniques.

Basic principles: Mass, volume, solutions, concentration, pH.
Preparation of solutions, buffers and use of the basic instrumentation of a laboratory.
Deionized water and MilliQ.
Spectroscopic techniques. Main theoretical principles: properties of electromagnetic radiation, interaction with matter.
UV and visible spectroscopy; theoretical principles, Lambert-Beer law, instrumentation, applications. Spectrofluorimetry, Colorimetry.
Chromatographic techniques: General principles.
Low pressure and high pressure chromatography (HPLC)
Molecular exclusion chromatography (Gel filtration)
Ion exchange chromatography
Affinity chromatography
Criteria for choosing a chromatographic system.
Electrophoretic techniques: General Principles
Support materials: Agarose, Polyacrylamide.
Protein electrophoresis: SDS-PAGE, electrophoresis in native conditions, Isoelectrofocusing (IEF), Two-dimensional electrophoresis (2-D PAGE)
Staining and revelation methods,
Western blotting.
DNA electrophoresis on agarose gel.
Capillary electrophoresis (outline).

Lecture.

Didactic material provided by the teacher

Oral examination.