MOLECULAR METHODOLOGIES FOR BIODIVERSITY CONSERVATION
Stampa
Enrollment year
2021/2022
Academic year
2021/2022
Regulations
DM270
Academic discipline
BIO/04 (PLANT PHYSIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
EXPERIMENTAL AND APPLIED BIOLOGY
Curriculum
Biologia ambientale e biodiversità
Year of study
Period
(01/10/2021 - 14/01/2022)
ECTS
6
Lesson hours
48 lesson hours
Language
Italian
Activity type
ORAL TEST
Teacher
BALESTRAZZI ALMA (titolare) - 6 ECTS
Prerequisites
Basic knowledge in cellular and molecular biology is required.
Learning outcomes
This part of the course aims at highlighting some methodological aspects of molecular biology applied to plant biodiversity issues, particularly as concerns GMOs traceability.
Course contents
GMOs (genetically modified organisms): definition, hystory. New-generation GMOs. GMOs and nutrition (in agreement with the 2030 Agenda for Sustainable Development, Goal 2, Zero Hunger) Environmental impact of transgenic plants. Transgene dispersal in soil and gene flow. Techniques for extraction and purification ot total DNA from soil, detection of recombinant DNA sequences by standar PCR (Polymerase Chain Reaction). Use of QRT-PCR (Quantitative RealTime-Polymerase Chain Reaction) for the detection and quantification of recombinant DNA in food and environment (GMOs traceability).
Teaching methods
Lectures.
Laboratory activities.
Reccomended or required readings
no text books are suggested but all the material will be provided by the teacher
Assessment methods
written exam
Further information
Sustainable development goals - Agenda 2030