2021/2022

2021/2022

DM270

MED/04 (GENERAL PATHOLOGY)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

EXPERIMENTAL AND APPLIED BIOLOGY

Bioanalisi

1°

2nd semester (01/03/2022 - 14/06/2022)

6

48 lesson hours

Italian

WRITTEN TEST

SCOTTI CLAUDIA (titolare) - 3 ECTS
FERRAROTTI ILARIA - 3 ECTS

Useful background knowledge comes from the contents of the course of General Pathology, particularly cellular pathology, inflammation and tumours. Basis of chemistry, physioogy and human anatomy are useful to follow the course, but for each chapter key information from other subjects is preliminarly revised.

The objective of the course is to introduce students to the integrated structure of a Clinical Pathology laboratory, analysing the chapters which are not covered by other courses and giving the student a through view of the field.

Introductory overview on the functioning of the immune system
Receptor repertoire of immune cells, cytokines, immunoglobulins, chemokines; production of antibodies, monoclonal antibodies, polyclonal antibodies.
The laboratory methodologies of the immunologist: isolation, cultivation and characterization of cells of the immune system; methods of immunization; detection and characterization of antibodies and their use in research and diagnostics; serological assays, affinity chromatography, radioimmunoassays, immunoenzymatic assays, competitive inhibition, hemagglutination, immunoprecipitation, cytotoxicity, neutralization; flow cytometry, tissue typing methods, functional assays; examples of laboratory diagnostics of some autoimmune diseases.
The transfusion laboratory; the HLA laboratory

Lectures

Kiro website, with extra material and links to deepen the knowledge.

Written exam, with three questions in the form of short assays.

Written exam, with three questions in the form of short assays.