2020/2021

2021/2022

DM270

MED/08 (PATHOLOGICAL ANATOMY)

DEPARTMENT OF MOLECULAR MEDICINE

BIOMEDICAL LABORATORY TECHNIQUES

PERCORSO COMUNE

2°

(04/10/2021 - 21/01/2022)

1

8 lesson hours

Italian

ORAL TEST

PAULLI MARCO (titolare) - 1 ECTS

1) To know and to define the principles of immunohistochemistry. 2) To know how to apply himmunoistochemistry in laboratory practice 3) To know how to apply immunohistochemistry to the breast pathology. 4) To be able to assess the quality and the efficacy of immunohistochemistry

• Introduction to immunohistochemistry
•Principles of fixation in immunohistochemistry
•Techniques of staininig in immunohistochemistry
•Immunofluorescence and immunoenzymatic
•Primary antibodies and antisera
•Antigenic demasking
•Standardization issues and automated immunohistichemistry
•The quality controls
•Diagnostic application of immunohistochemistry
•The role of immunohistochemistry in the diagnosis and prognostication of pre-neoplastic and neoplastic lesions of the breast
•Presentation of some paradigmatic cases

Lectures. Professor is available for clarification on the topics of the program.

Robbins, Pathological Bases of Diseases.

Oral exam with multiple questions aimed at ascertaining the knowledge acquired on immunoistochemistry and its diagnostic issues, including etiopathologic and clinical correlations. The assessment will also take into account the ability to communicate and use an appropriate scientific language.