2020/2021

2020/2021

DM270

MED/07 (MICROBIOLOGY AND CLINICAL MICROBIOLOGY)

DEPARTMENT OF MOLECULAR MEDICINE

BIOMEDICAL LABORATORY TECHNIQUES

PERCORSO COMUNE

1°

(01/03/2021 - 18/06/2021)

2

16 lesson hours

Italian

ORAL TEST

SPALLA MELISSA - 2 ECTS

-

The aim of the course is to provide the student with the essential knowledge to understand the principles and methods of execution of the main methodologies for laboratory diagnosis of infections.
In particular, the course aims to:
- provide adequate knowledge on the proper management of the biological sample;
- provide adequate specific knowledge of methods for evaluation of correct antibiotic susceptibility ;
- provide adequate knowledge on the main methods of identification and bacterial typing.
At the end of the course the student is expected to have achieved the necessary knowledge to apply a correct and effective diagnostic process.

- Sampling of clinical specimens (from various anatomical districts) for microbiological analysis and their proper conservation
- The microbiological sample: processing priority, acceptability.
- Baterial count.
- Direct and indirect diagnostic tools by using microscopy, coltural with manual / automated systems. Molecular methods, i.e. PCR (gene amplification) and immunology.
- Innovations in the field of bacterial identification: automated systems, mass spectrometry, molecular methods.
- In vitro antimicrobial activity evaluation (antibiogram, MIC, and MBC). Synergy test, combined test, and commercial methods for diagnostic confirmation of ESBL and / or carbapenemases production. Colistin resistance.
- Multi-resistance concept, extended resistance, pan-resistance.
- Characterization of bacterial isolates and their storage in the laboratory.
- Nosocomial infections and epidemic events (definitions): phenotypic and genotypic typing methods.

The topics concerning the contents of the course will be illustrated and discussed through frontal teaching.

Didactic material provided by the teacher;
Text:"Microbiologia clinica. Con Contenuto digitale (fornito elettronicamente)" by Eudes Lanciotti. Publisher: CEA, fourth edition, 2017.
or
text: "Microbiologia clinica. Per i corsi di laurea in medicina e chirurgia e in professioni sanitarie" author Roberto Cevenini; Publisher Piccin-Nuova Libraria

Oral examination

-