2020/2021

2021/2022

DM270

CHIM/06 (ORGANIC CHEMISTRY)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

BIOTECHNOLOGY

PERCORSO COMUNE

2°

(01/10/2021 - 14/01/2022)

3

24 lesson hours

Italian

ORAL TEST

PORTA ALESSIO (titolare) - 3 ECTS

The student is required to have knowledge of general chemistry and organic chemistry at the level of basic courses and of physics at the level of high school programs.

Module seeks to provide a basic understanding of the main types of techniques and instruments used in mass spectrometry of biomolecules, as well as a basic familiarity with interpreting their data. At the end of the course it is expected that the student will be able to understand the various spectroscopic MS techniques used on molecules of biological interest and to be able to evaluate their application.

Techniques in organic mass spectrometry.
Basic components and processes of ionisation, separation, detection. Formation of the mass spectrum: molecular ion, isotopic peaks, problems from isotopic clusters in biomolecules. Basic mechanisms of ion fragmentation: cleavage of a bond on an atom adjacent to the atom bearing the odd electron, reaction through electron withdrawal by the charge site. Ionisation techniques: Electron Ionisation (EI); Chemical Ionisation (CI), Fast Atom Bombardment (FAB), Matrix-Assisted Laser Desorption Ionisation (MALDI): principles, practical considerations on biological mass spectrometry, direct imaging, clinical applications. Atmospheric pressure ionisation: Electrospray Ionisation (ESI), Atmospheric-Pressure Chemical Ionisation (APCI) and other techniques in ambient mass spectrometry. Tandem MS (MS/MS): ion activation (CID etc) and instrumentation. The coupling of mass spectrometry with liquid chromatography (LC-MS/MS). Protonation of a dipeptide results in cleavage of the amide bond: notation for indicating peptide fragments that arise from a mass spectrum. The model of the mobile proton to rationalize the fragmentation of peptides.

Lectures. During the lessons of the mass spectrometry module, spectroscopic data will be projected in the classroom and analyzed and discussed by the teacher. The teacher is obviously available for clarifications on the topics covered in class.

E. De Hoffmann, V. Stroobant, Mass Spectrometry: Principles and Applications, 3rd Edition, Wiley, 2007, ISBN: 978-0-470-03311-1.
Handouts and lecture slides are available from the teacher.

The module requires passing a written exam to questions with open answers on theory, techniques of mass spectrometry and spectra interpretation.

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