BIOCHEMISTRY
Stampa
Enrollment year
2020/2021
Academic year
2021/2022
Regulations
DM270
Academic discipline
BIO/10 (BIOCHEMISTRY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
BIOTECHNOLOGY
Curriculum
PERCORSO COMUNE
Year of study
Period
1st semester (01/10/2021 - 14/01/2022)
ECTS
9
Lesson hours
72 lesson hours
Language
Italian
Activity type
ORAL TEST
Teacher
LAVATELLI FRANCESCA (titolare) - 3 ECTS
TORTI MAURO - 6 ECTS
Prerequisites
Good knowledge on General and Organic Chemistry
Learning outcomes
The aims include: the knowledge of the structure and function of the major macromolecules of biological interest with particular attention to the structural-functional relationships; the understanding of the metabolic processes and energetic pathways in the living cell and the mechanism for regulation and integration of the metabolism; the understanding of the mechanisms and signal transduction pathways for cellular communication
Course contents
Part 1. Structural and chemical features of amino acids. Peptides. Methods for analysis of protein biochemistry. Three dimensional structure of proteins. Collagens and immunogloblins. Proteins in oxygen binding and transport: myoglobin and hemoglobin. Enzymes. Mechanisms of catalysis. Vitamins and coenzymes. Enzyme kinetics and mechanisms of regulation. Allosteric enzymes. Monosaccharides and polysaccharides. Proteoglycans and glycoproteins. Structural lipids and storage lipids. Lipids in the biological membranes. Membrane proteins: structure and function.

Part 2. Principles of bioenergetics. The role of ATP and phosphate group transfers. The importance of biological oxidations. The citric acid cycle. Oxidative phosphorylation and ATP synthesis. The chemiosmotic model. Regulation of mitochondrial function and ATP synthesis. Carbohydrate metabolism. Glycolysis. Gluconeogenesis. Glycogen metabolism. Shunt of pentose phosphate. Regulation of glucose metabolism in the liver and muscle under aerobic and anaerobic conditions.. Lipids metabolism. b-oxidation of fatty acids. The ketone bodies. Biosynthesis of fatty acid. Biosynthesis of fatty acid, triacilglicerols and phospholipids. Regulation of lipids metabolism. Amino acid catabolism. Transaminases. The urea cycle. General concepts on amino acids degradation. Hormonal regulation of fuel metabolism. insulin and glucagon. Molecular mechanisms of signal transduction.
Teaching methods
lectures in the classroom
Reccomended or required readings
Nelson DL, COX, MM : I Principi di Biochimica di Lehninger, Zanichelli;
Berg JM, Tymoczko JL, Stryer L: Biochimica, Zanichelli;
Campbell, Farrell: Biochimica, Edises,
Bassi R, Boffi A, et al: Biochimica, Edi-Ermes
Assessment methods
Verbal exam
Further information
Verbal exam