Enrollment year
2020/2021
Academic discipline
BIO/10 (BIOCHEMISTRY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
ADVANCED BIOTECHNOLOGY
Curriculum
PERCORSO COMUNE
Period
2nd semester (01/03/2021 - 14/06/2021)
Lesson hours
48 lesson hours
Prerequisites
Basic knowledge of biochemistry and molecular biology. Knowledge of the general concepts about protein structure and function and cell metabolism.
Learning outcomes
The students must acquire the knowledge and the ability to describe the techniques and the strategies for the identification, the production and the characterization of recombinant enzymes, and for their uses in industry. THe students will also be able to describe the scientific and technical basis of monoclonal antibodies and recombinant proteins and their use as therapeutics and advanced diagnostics and a new class of drugs based on cells, so called Advanced Therapy medicinal products, that are contributing to the revolution of medicine of the future.
Course contents
Enzymes: structure, catalytic properties and mechanisms, elements of enzyme kinetic. Biochemistry of industrial enzymes: production, purification and characterization of proteins and enzymes. Methods and techniques for protein purification: centrifugation, dialysis, chromatography, electrophoresis. Measure of enzyme catalytic activity, protein quantification, specific activity. Enzyme assays and immunoassays. Enzymes in analytical biochemistry and diagnostics. Main families of industrial enzymes: biochemical features and relevance in industry. Practical applications of enzymes in the textile industry, in detergency and in the agro-food industries.
Monoclonal Antibodies: scientific and technical basis , generation, production, characterization, therapeutic and diagnostic applications. Enzyme Immuno Assay (ELISA), labelled antibodies for in vitro and in vivo diagnostics. Recombinant proteins : scientific and technical basis, development and production, purification, therapeutic and diagnostic applications. Cell-based drugs: Advanced Therapy medicinal products (cell and gene therapy, tissue engineering). Development and production, compliance with European regulation, therapeutic applications in patient specific therapy and personalized medicine.
Teaching methods
Frontal lessons, seminars held by experts in the field of the biotechnology, guided tours of production plants.
Reccomended or required readings
Enzymes in Industry: Production and Applications. 2007. Wolfgang Aehle (Ed). John Wiley & Sons.
Monoclonal antibodies: versatile platforms for cancer immunotherapy. Nature Reviews Immunology 10, 317-327 (May 2010)
Therapeutic antibodies: past, present and future. Nature Reviews Immunology 10, 297 (2010)
Strategies for the Production of Recombinant Protein in Escherichia coli. Gopal Jee Gopal • Awanish Kumar Springer Science+Business Media New York 2013
Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications. Biotechnology Journal :620-34. 2012
Course notes and material provided by the teacher.
Assessment methods
Oral exam to determine student's learning abilities and fluency in presenting scientific topics.
Sustainable development goals - Agenda 2030
Università di Pavia - Offerta formativa