MICROSCOPIC AND CITOCHEMISTRY TECHNIQUES
Stampa
Enrollment year
2020/2021
Academic year
2020/2021
Regulations
DM270
Academic discipline
BIO/06 (COMPARATIVE ANATOMY AND CYTOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
EXPERIMENTAL AND APPLIED BIOLOGY
Curriculum
Bioanalisi
Year of study
Period
1st semester (05/10/2020 - 14/01/2021)
ECTS
6
Lesson hours
48 lesson hours
Language
Italian
Activity type
ORAL TEST
Teacher
BIGGIOGERA MARCO (titolare) - 3 ECTS
MILANESI GLORIA ANGELA - 3 ECTS
Prerequisites
=
Learning outcomes
Knowledge of basic and advanced microsopy techniques and of cytochemical methods
Course contents
Microscopes: bright field, dark field, polarization, interference, DIC, fluorescence (with basic notions), confocal, trasmission electron microscope, scanning electron microscope, energy filtering electron microscope. Resolution and contrast. Preparative methods of biological samples for light and electron microscopy: fixation, dehydration, embedding in resin and paraffin. Staining for for light and electron microscopy. Immunocytochemistry: mono- and polyclonal antibodies; direct and indirect reactions; elctrondense and fluorescent markers. In situ hybridization: different probe types, stringency limits and specificity; applications. Cytochemical reactions: concept of reaction specificity or preferentiality: Reactions for DNA: Feulgen reaction; osmium ammine; specific fluorochromes. Flow cytometry: basics and applications. Some examples of application of cytochemical and microscopical techniques for the detection of apoptosis and necrosis. New dynamical techniques: FRAP, FLIP, iFRAP. Superresolution: scanning tunneling microscope and atomic force microscope. Stimulated Emission Depletion (STED), Photoactivated Localization Microscopy (PALM), Stochastic optical reconstruction microscopy (STORM). Electron tomography and 3D reconstruction of objects. Correlative microscopy. Enzyme histochemistry: basics and applications.
Teaching methods
Lectures and practical activity with fluorescence and electron microscopes
Reccomended or required readings
=Chandler & Roberson:BIOIMAGING. Jones & Bartlett Publishers
Assessment methods
Oral exam
Further information
Oral exam
Sustainable development goals - Agenda 2030