2020/2021

2020/2021

DM270

BIO/19 (GENERAL MICROBIOLOGY)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

EXPERIMENTAL AND APPLIED BIOLOGY

Bioanalisi

1°

2nd semester (01/03/2021 - 14/06/2021)

9

72 lesson hours

Italian

WRITTEN TEST

PASCA MARIA ROSALIA (titolare) - 6 ECTS
BURONI SILVIA - 3 ECTS

Knowledge of General Microbiology provided by the Degree Courses in Biological Sciences and in Biotechnology.

Knowledge of methodologies for microbiological control of food, water, and health products.

Knowledge of the characteristics of the main pathogen microorganisms (bacteria and viruses) in the clinical field and of the techniques that allow their identification.

Part 1. Intrinsic and extrinsic parameters that affect microbial growth and survival in foods. Determining microorganisms and/or their products: culture, microscopic, and sampling methods; chemical, biological, and physical methods; bioassays and related methods. Foodborne diseases. Examples of food analyses (meet, eggs, milk). Microbial analysis of cosmetics. Water microbiology. Monitoring of air and surface microbial quality. Traceability of genetically modified organisms.

Part 2. Bacterial and viral pathogens of humans: pathogenesis and clinical features. Antibiotics and vaccins. Main conventional diagnostic techniques: staining; processing of different clinical specimens (urine, blood, various swabs, respiratory samples, cerebrospinal fluid, feces, etc.); physiological, serological and antigenic tests used to identify the bacteria; antibiogram; etc. Molecular diagnostic techniques used to detect non-culturable pathogenic bacteria and viruses.

The course is divided into frontal lessons.

- Madigan MT, Martinko JM, Stahl DA, Clark DP. Microbiologia biomedica 3. BROCK. Biologia dei microrganismi. 2012 Pearson, Milano.
- G. Antonelli, M. Clementi, G. Pozzi, G.M. Rossolini. 2008. Principi di Microbiologia Medica. Casa Editrice Ambrosiana. Milano
- Eudes Lanciotti. Principi di Microbiologia Clinica. 2007. Casa Editrice Ambrosiana, Milano.
- S.G. Gillespie, P.M. Hawkey. 2007. Principles and Practice of Clinical Bacteriology.
Second edition. John Wiley and Sons Ltd. West Sussex, England, UK.
- Pavone, Paolucci. Biologia e Microbiologia dell'ambiente e degli alimenti. Zanichelli.
-Galli Volonterio. Microbiologia degli alimenti. Casa Editrice Ambrosiana.
-Microbiologia dei prodotti alimentari. Casa Editrice Ambrosiana.
- Didactic material provided by teachers (Kiro web site).

The examination is written, with 9 open questions to verify the study and the knowledge of the student. The duration of the test is 2h 30'.

The examination is written, with 9 open questions to verify the study and the knowledge of the student. The duration of the test is 2h 30'.