DIAGNOSTIC METHODOLOGY IN CLINICAL MICROBIOLOGY
Stampa
Enrollment year
2019/2020
Academic year
2021/2022
Regulations
DM270
Academic discipline
MED/07 (MICROBIOLOGY AND CLINICAL MICROBIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
BIOTECHNOLOGY
Curriculum
PERCORSO COMUNE
Year of study
Period
2nd semester (01/03/2022 - 14/06/2022)
ECTS
6
Lesson hours
48 lesson hours
Language
Italian
Activity type
WRITTEN TEST
Teacher
NUCLEO ELISABETTA (titolare) - 3 ECTS
ZARA FRANCESCA - 3 ECTS
Prerequisites
It is recommended to have passed the 2nd year General Microbiology exam. In order to better follow the course, the student should have attended the Histology, Human Anatomy, Biochemistry, Physiology and Genetics courses.
Learning outcomes
Diagnostic methodology (3 CFU)
The course provides the students with the knowledge about the phenotypic susceptibility testing and molecular methods used in Microbiology laboratories to type Gram-positive and Gram-negative bacteria.

Clinical Microbiology (3 CFU)
The course aims to provide students with the cognitive and methodological tools necessary to understand the host-pathogen interaction, the etiological diagnosis of infections of the various apparatuses and systems of the human organism and therapeutic and preventive strategies.

At the end of the integrated course, the student should be able to know how to analyse and apply the knowledge and skills acquired during the lectures to the understanding
of the pathogenetic mechanisms of the various microorganisms, of the main diseases contracted, and of the latest diagnostic tests of infections.
Course contents
Diagnostic Methodology (3 CFU)
Introduction to the course
Microbiological diagnostics of antibiotic-resistance
Clinical outcome of antibiotic-resistance mechanisms
Epidemiology and characterization of Extended-Spectrum- beta-Lactamases (ESβL) emerging in nosocomial Gram-negative pathogens
Class A, B, C and D ESβL
Methods of bacterial genotyping
Beta-lactams, aminoglycosides, macrolides e fluoroquinolones
New antimicrobial agents
Sequencing
Sequences analysis
Healthcare-associated infections.

Clinical Microbiology (3 CFU)
Introduction to the course
Microbiota of the human body in normal and pathological conditions.
Host-pathogen interactions.
Microbiological characteristics, causative agents, pathogenetic mechanisms, clinical presentations, microbiological diagnosis and treatment of:
central nervous system infections,
sepsis and cardiac infections,
respiratory tract infections,
gastrointestinal tract infections,
genitourinary tract infections, infections of the fetus and newborn.
Teaching methods
The course is split into 2 integrated modules, Diagnostic Methodology (3CFU) and Clinical Microbiology (3CFU) and includes lectures.

Teachers are available for clarifications about the topics covered in class, by appointment to be booked (via email).
Reccomended or required readings
Cevenini - "Microbiologia Clinica" - Editore Piccin.

"Microbiologia medica"
Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller - Editore Edra Masson.

Educational material provided by the teachers.
Assessment methods
The examination of the integrated course consists of a written test (duration: 1.5 hours), structured in 6 open questions related to the main topics covered during the course.
Further information
The examination Commission consists of Prof. Francesca Zara and Prof. Elisabetta Nucleo.
Sustainable development goals - Agenda 2030
Goal 1: No poverty
Goal 2: Zero hunger
Goal 3: Good health and well-being
Goal 4: Quality education
Goal 5: Gender equality
Goal 6: Clean water and sanitation
Goal 7: Affordable and clean energy
Goal 8: Decent work and economic growth
Goal 9: Industry, innovation and infrastructure
Goal 10: Reduced inequalities
Goal 11: Sustainable cities and communities
Goal 12: Responsible consumption and production
Goal 13: Climate action
Goal 14: Life below water
Goal 15: Life on Land
Goal 16: Peace, justice and strong institutions
Goal 17: Partnerships for the goals
The goals