DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
1st semester (05/10/2020 - 14/01/2021)
72 lesson hours
Good knowledge on General and Organic Chemistry
The aims include: the knowledge of the structure and function of the major macromolecules of biological interest with particular attention to the structural-functional relationships; the understanding of the metabolic processes and energetic pathways in the living cell and the mechanism for regulation and integration of the metabolism; the understanding of the mechanisms and signal transduction pathways for cellular communication
Part 1. Structural and chemical features of amino acids. Peptides. Methods for analysis of protein biochemistry. Three dimensional structure of proteins. Collagens and immunogloblins. Proteins in oxygen binding and transport: myoglobin and hemoglobin. Enzymes. Mechanisms of catalysis. Vitamins and coenzymes. Enzyme kinetics and mechanisms of regulation. Allosteric enzymes. Monosaccharides and polysaccharides. Proteoglycans and glycoproteins. Structural lipids and storage lipids. Lipids in the biological membranes. Membrane proteins: structure and function.
Part 2. Principles of bioenergetics. The role of ATP and phosphate group transfers. The importance of biological oxidations. The citric acid cycle. Oxidative phosphorylation and ATP synthesis. The chemiosmotic model. Regulation of mitochondrial function and ATP synthesis. Carbohydrate metabolism. Glycolysis. Gluconeogenesis. Glycogen metabolism. Shunt of pentose phosphate. Regulation of glucose metabolism in the liver and muscle under aerobic and anaerobic conditions.. Lipids metabolism. b-oxidation of fatty acids. The ketone bodies. Biosynthesis of fatty acid. Biosynthesis of fatty acid, triacilglicerols and phospholipids. Regulation of lipids metabolism. Amino acid catabolism. Transaminases. The urea cycle. General concepts on amino acids degradation. Hormonal regulation of fuel metabolism. insulin and glucagon. Molecular mechanisms of signal transduction.
lectures in the classroom
Reccomended or required readings
Nelson DL, COX, MM : I Principi di Biochimica di Lehninger, Zanichelli;
Berg JM, Tymoczko JL, Stryer L: Biochimica, Zanichelli;
Campbell, Farrell: Biochimica, Edises,
Bassi R, Boffi A, et al: Biochimica, Edi-Ermes
Sustainable development goals - Agenda 2030