2019/2020

2019/2020

DM270

BIO/11 (MOLECULAR BIOLOGY)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

PERCORSO COMUNE

1°

1st semester (01/10/2019 - 14/01/2020)

6

48 lesson hours

English

WRITTEN TEST

GIULOTTO ELENA (titolare) - 4.5 ECTS
GIULOTTO ELENA (titolare) - 1.5 ECTS
RUIZ HERRERA MORENO AURORA MANUELA - 1.5 ECTS

Basic concepts in Molecular Biology, Genetics and Biochemistry

The objective of the course is to provide knowledge in advanced molecular biology concepts and methods, including genomics, post-genomics and epigenetics. In addition, students will learn the english terminology in the field. Through journal clubs and scientific paper reading with the teacher, students will learn to critically evaluate the scientific literature in the field. Students will be able to apply the knowledge acquired in the most advanced molecular biology methods to their laboratory practice and research activity.

Specific subjects include: organization of different genomes; DNA markers; genome maps; sequencing of entire genomes; next generation sequencing methods and applications; genome sequence interpretation (sequence analysis, experimental approaches, gene identification); defining gene function (gene inactivation, RNAi); genome modification by engineered nucleases; chromosome architecture (chromatin, centromeres, telomeres); comparative genomics; molecular mechanisms of genome evolution. Epigenetics. Model organisms. Production of recombinant proteins.

Lectures, Journals clubs, Discussions with students, problem solving

"Genomes 3", TA Brown, Garland Science Publishing

"From genes to genomes, Concepts and applications of DNA technology", JW Dale, MV Schantz, N Plant, third edition, Wiley-Blackwell

"Gene cloning and DNA analysis, An Introduction" Fifth edition, TA Brown, Blackwell Publishing

Written exam icluding open questions on general topics and multiple choice questions.

Written exam icluding open questions on general topics and multiple choice questions.