2019/2020

2021/2022

DM270

BIO/18 (GENETICS)

DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"

BIOLOGICAL SCIENCES

PERCORSO COMUNE

3°

2nd semester (01/03/2022 - 14/06/2022)

6

48 lesson hours

Italian

ORAL TEST

RAIMONDI ELENA MARIA CLOTILDE (titolare) - 6 ECTS

Basic knowledge of Genetics and Molecular Biology.

Structure and organization of the genome of higher eukaryotes. Epigenetic regulation of gene expression. Introduction to molecular cytogenetics.

Structure and organisation of the genome of bacteria and bacterial viruses. Structure and organisation of the eukaryotic genome. The prokaryotic gene and the operon concept. Evolution of the concept of gene. The C value and its paradox. Single copy and repeated DNA sequences. Functions of repeated DNA. Gene families, moderately repeated DNA, low copy repeats (LCR sequences and CNV), highly repeated DNA sequences, satellite DNA, interspersed repeated DNA sequences. Transposable elements. Eukaryotic transposons. Transposable elements, LCR sequences and genome evolution. The nucleosome. Chromatin in its functional state. Genetic maps. Physical maps. Construction of physical maps in man: pedigree analysis, somatic cell hybridisation, radiation hybrids, in situ hybridisation. Regulation of gene expression in eukaryotes: epigenetic regulation of gene expression. Polytenic chromosomes and puffing. Lamp-brush chromosomes. Gene dosage compensation and sex determination. Genomic imprinting. Dissection of the eukaryotic chromosome: centromere, telomere and replication origins. The epigenetic nature of centromere function. Reconstruction of the eukaryotic chromosome: mammalian artificial chromosomes. Gene therapy. Stem cells. Cloning of organisms.

Lectures

Reference books:
PRINCIPI DI GENETICA - Peter D. Snustad, Michael J. Simmons - Curato da: Gaudio L., Polito C. – Edises (4a edizione)
GENETICA (Principi di analisi formale) – Antony J.F. Griffiths, Susan R. Wessler, Sean B. Carrol, John Doebley – Zanichelli (7° edizione)
EREDITÀ. PRINCIPI E PROBLEMATICHE DELLA GENETICA UMANA - Cummings MR - EdiSES, 2009
BIOLOGIA MOLECOLARE DELLA CELLULA - Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P: Quinta edizione (2008) - Zanichelli. ISBN 978-88-08-06451-6
IL GENE - Lewin, Krebs, Goldstein, Kilpatrick -Zanichelli Ed. - Seconda edizione compatta, 2011
Students also will be delivered scientific reviews on specific topics and all the slides of the course.

The exam is oral and consists of query on the entire program.

The exam is oral and consists of query on the entire program.