Enrollment year
2017/2018
Academic discipline
BIO/13 (APPLIED BIOLOGY)
Department
DEPARTMENT OF BIOLOGY AND BIOTECHNOLOGY "LAZZARO SPALLANZANI"
Course
BIOLOGICAL SCIENCES
Curriculum
PERCORSO COMUNE
Period
1st semester (01/10/2019 - 14/01/2020)
Lesson hours
72 lesson hours
Activity type
WRITTEN TEST
Prerequisites
Basics of Genetics, Cytology and Histology.
Learning outcomes
Basic knowledge of microscopy and cytogenetics.
Course contents
Module 1. Basic principles of optical microscopy. Main types of optical microscopy (transmitted light, reflected light, dark field, polarization, phase contrast and interference, fluorescence) and their fields of application. Outline of confocal microscopy, two-photon microscopy and atomic force microscopy. The fluorescence phenomenon as photo-physical spectra of excitation / emission, quantum efficiency and decay time. Fluorimetric techniques in the study of cells and tissues: natural fluorescence and fluorescence induced. The fluorophores as markers of cellular structures and functions. Immunofluorescence techniques.
Module 2. In vitro cultures of mammalian somatic cells. In vitro cultures from peripheral blood. Chromosome preparations. Chromosome banding. The normal and pathological human karyotype. Reconstruction of the human karyotype. Differential staining of sister chromatids: SCE. Labelling of DNA probes: nick-translation. Fluorescence in situ hybridization. Probes evidentiation. Analysis of the experimental results by fluorescence microscopy. Use of high definition camera (CCD). Acquisition and processing of digital images.
Teaching methods
Educational workshops
Reccomended or required readings
Dispenses with the slides presented during the course.
Assessment methods
Written test on the experiments carried out.
Further information
Written test on the experiments carried out.
Sustainable development goals - Agenda 2030
Università di Pavia - Offerta formativa