2016/2017

2018/2019

DM270

BIO/12 (CLINICAL BIOCHEMISTRY AND BIOLOGY)

DEPARTMENT OF MOLECULAR MEDICINE

BIOMEDICAL LABORATORY TECHNIQUES

PERCORSO COMUNE

3°

(01/10/2018 - 11/01/2019)

3

24 lesson hours

Italian

ORAL TEST

NUVOLONE MARIO ULISSE - 2 ECTS
PALLADINI GIOVANNI - 1 ECTS

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The student:
1) Will know the main techniques of molecular biology for the extraction, amplification and analysis of nucleic acids
2) Will know the main applications of molecular diagnostics

1. Nucleic acid extraction techniques
2. Nucleic acid quantification techniques
3. Nucleic acid quality control
4. Nucleic acid amplification techniques: basic principles, main types
5. Polymerase chain reaction: basic principles, assay design, technical considerations, main applications
6. PCR variants: Hot start PCR, Touch down PCR, Nested PCR, Multiplex PCR, Inverse PCR, Direct PCR, GC-rich PCR, Reverse transcriptase polymerase chain reaction (RT-PCR), Quantitative polymerase chain reaction (qPCR), real time qPCR, Reverse transcriptase qPCR (RT-qPCR), digital PCR
7. Nucleic acid electrophoresis
8. Restriction Fragment Length Polymorphism (RFLP)
9. PCR Restriction Fragment Length Polymorphism (PCR-RFLP)
10. High resolution melting analysis
11. Microarrays: SNV chips, gene expression microarrays, Arrays for comparative genomic hybridization (aCGH)
12. In situ hybridization: Fluorescence in situ hybridization (FISH), Spectral karyotyping (SKY)
13. Dideoxy-Termination Sequencing (Sanger Sequencing)
14. Massively parallel sequencing: basic principles
15. Bridge amplification and reversible dye terminator sequencing
16. Real-Time single molecule sequencing with fluorescent nucleotides
17. Target-enrichment strategies for massively parallel sequencing
18. Whole-exome sequencing (WES)

• Frontal lectures

Laboratory case discussion

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics

Written exam

Written exam