2021/2022

2021/2022

DM270

BIO/13 (APPLIED BIOLOGY)

DEPARTMENT OF MOLECULAR MEDICINE

MEDICAL AND PHARMACEUTICAL TECHNOLOGIES

PERCORSO COMUNE

1°

(28/02/2022 - 10/06/2022)

6

48 lesson hours

Italian

WRITTEN TEST

GIORGIO ELISA - 3 ECTS
SOTTILE VIRGINIE - 3 ECTS

Knowledge of the biological mechanisms related to the cell cycle (including mitosis and meiosis-spermatogenesis and oogenesis); embryogenesis; DNA structure and replication; transcription; translation; apoptosis; repair mechanisms.

Biology: the student must know the basic mechanisms at a deeper level. Particular attention will be given to the regulation of the Cell Cycle and to the regulation of gene expression. The student must also be able to support and compare notions in the different areas of biology, finding common matrices.

Stem cells. The student will have to know the basic characteristics of stem cells (SC), compare these characteristics according to the origin of the cells (eg Embryonic Stem Cells vs Adult Stem Cells). They will also have to know the related technologies and therapeutic applications of SC, clearly describing their limits. Particular attention will be given to preclinical models used to study SC. Finally, the role of SC in pathologies, including Cancer SC, will be discussed.

BIOLOGY
Cell: Differences between prokaryotic and eukaryotic cell. Cell organelles and their function
Cell cycle and its regulation.
Structure of Nucleic Acids. The DNA as genetic material. Transformation: the Griffith and Avery’s experiment. Hershey and Chase’s experiment. DNA replication. Telomeres and telomerase.
Extra nuclear DNA: mitochondria, chloroplasts
Cell division: mitosis and meiosis. Spermatogenesis and oogenesis. Fertilization.
The central dogma of Biology.
RNA types and functions. Transcription. The maturation of mRNA in eukaryotes.
Translation.
The Genetic Code.
Acquisition of three-dimensional structure of proteins.
Regulation of gene expression. From the LAC operon in prokaryotes to miRNA.

STEM CELLS
- General characteristics
- How Stem cells can be obtained (ESC, iPSC, Adult SC)
- Preclinical models and SC applications
- SCs & tissue regeneration
- Stem Cells and Cancer
- developing therapeutic approaches

Lectures supported by Powerpoint presentations. For some topics, blackboard and youtube animations will be also used.

Biology
Molecole, Cellule e Organismi di Ginelli, Malcovati et al. Edises
Biologia Cellulare e Molecolare di G. Karp Edises
Biologia e Genetica di De Leo, Ginelli, Fasano Edises
Biologia Molecolare della cellula di B. Alberts et al Zanichelli
GENETICA di D. L. Hartl e E. W. Jones. Edises
iGENETICA di P.J. Russel Edises

STEM CELLS
Articles and reviews from international scientific journals selected by the teacher

The exam consists of two written tests

BIOLOGY
Multiple choice questions and open questions. The questions will focus on the topics covered in class.

STEM CELLS
Multiple choice questions and open-ended questions.
The student will have to demonstrate not only the knowledge of the topics covered but also the ability to link them.

For communications and appointments with the teacher please use the following e-mail address:

BIOLOGY elisa.giorgio@unipv.it

STEM CELLS virginie.sottile@unipv.it